ICAM1 antibody

Catalog No. ABIN206913

This anti-ICAM1 antibody is a Mouse Monoclonal antibody detecting ICAM1 in WB, FACS, IHC, ICC and IHC (fro). Suitable for Human.

Quick Overview for ICAM1 antibody (ABIN206913)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1H4

Product Details anti-ICAM1 Antibody

Specificity: Reacts with CD54 (ICAM-1), a 85-110 kD type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation, it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

Purification: Protein A purified

Immunogen: Raji cells and spleen cells fused with NS1 cells.

Type of Immunogen: Cells

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo, ICC, IHC, IHC-Fr, WB

Usage: Flow Cytometry: Recommended dilution - 1 μg/mL. Immunohistochemistry (paraffin sections): Recommended dilution - 10 μg/mL. Application Note - Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Azide free PBS, preserved by filter sterilization, approx,  pH 7.4.

Preservative: Azide free

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: ICAM1 / CD54

Background: Name/Gene ID: ICAM1
Family: ICAM

Synonyms: ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58

Gene ID: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling